Author Archives: sungyonkim@gmail.com

Prof. Liqun Luo visits the Kim lab

Prof. Liqun Luo, a leading neuroscientist at Stanford, visited the Kim lab and had a fun time chatting with the students over a cup of tea. He also kindly signed his autograph on his new neuroscience textbook. Hope to see him more often as we grow into a “good” neuroscience lab!

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The K-Lab welcomes Han-Eol, Dong-Jun, Minjin as PhD students

Han-Eol, Dong-Jun and Minjin, who have been working in the lab since the lab started, formally joined the K-Lab as PhD students. They are now in either School of Biological Sciences or Interdisciplinary Program in Neuroscience. These outstanding students have been doing super well, but now it is a whole another beginning. Cannot wait to see them blossoming as excellent neuroscientists!

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Our first paper is published in Molecules and Cells

Our first paper (a review paper on tissue clearing and labeling techniques) is now online in Molecules and Cells website. A small contribution to the tissue clearing field and to the Korean Society for Molecular and Cellular Biology. Hope you find this review useful and cite when discussing tissue clearing/labeling! And great job, Jinyoung and Minjin!

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Fig. 1. Tissue clearing techniques. (A) Light scattering in biological tissues can be reduced by removal of lipid and RI matching. (B) Simple immersion in a high-RI aqueous solution renders the tissue modestly transparent by homogenizing scattering throughout the sample. (C) Delipidation and dehydration / hyperhydration followed by refractive index matching. (Top) For solvent-based clearing, the tissue is incubated in dehydrating solvent for delipidation and dehydration, and is moved to a high-RI clearing solvent where RI matching and additional delipidation occur. (Bottom) The sample is placed in an aqueous solution that contains high concentration of non-ionic detergent and denaturant, where delipidation, hyperhydration, and RI matching take place. (D) A biological sample is first transformed into a tissue-gel hybrid by hydrogel embedding (Top) or glutaraldehyde fixation (Bottom), where the gel network increases the tissue integrity. The tissue-gel hybrid then can withstand extensive delipidation by incubation in ionic detergent (SDS) assisted by electrophoresis or heating.